To collect macromolecular crystallography data in 5ID-B the beam transport pipe must first be in place. The beam pipe allows us to minimize the air in the path of the X-ray beam. Two pipes are used for the mar165: a short extension metal pipe (labeled “… mar165 1”) + a longer PVC pipe (labeled “… mar165 2”).
If you are switching over from users in 5ID-C or 5ID-D, follow the instructions in mar165_protocol. Do not do this switch unless you have been trained to do so.
An ion chamber is installed in 5ID-B downstream of the slit tank, near the
front of the hutch (see PF2S2 for picture). Check
the ion chamber connections as per this figure:
The PS2S2 / PF4 assembly should be immediately downstream of the ion chamber
as shown in this figure.
In protoscan this assembly is referred to as the PF2S2 and contains an X-ray
shutter and four filters. Currently only 4 of the filters, containing
Al plates of varied thickness, are used
The Mar table is the bread-board table on wheels at the back of the 5ID-B hutch. This table contains the Mar165 base, the Mar165 CCD detector, the Cryojet, and the Amptek detector. The table itself is aligned with the beam. The equipment is mounted on sliders which allow it to be translated out of the beam path without losing the alignment as per the mar165_protocol.
The Cryojet is the Oxford system for keeping crystal samples at low temperatures
during data collection. It is mounted on a slider with the Amptek detector
on the Mar table. The controller for the Cryojet is under the Mar table.
When not in use, the rates for the cold “sample flow” (inner tube)
and the warm “shield flow” (outer tube) are turned down to
2.5 and 2.5 as shown in this figure.
this causes the sample temperature to rise above its set point. During
use, the flows are adjusted to 7.0 – 7.5 for the sample flow and 5.5 –
6.0 for the shield flow. The flow and temperature are set by pressing
and holding the respective black “set” button and adjusting the
level with the red “raise” and “lower” buttons.
The Cryojet should be aligned collinear with the spindle axis. This is
best done from the backside of the mar table with a second person checking the
alignment from the sample side.
If you will be collecting MAD data, you will need the Amptek detector to select
your edge energy. A step-by-step wiring checklist for this detector is
available on the beam-line. You can confirm the wiring by comparing it
to the diagram below (also available on the beam-line).
There are several video cameras in the 5ID-B hutch. These are connected with BNC cables through the patch panel to the ProVideo Auto switch on the outside of the hutch. The current camera configuration is as follows (referring to the channel numbers on the ProVideo switch):
1.
crystal
located in the mar base underneath the crystal
2.
Cryojet nozzle
on the Cryojet slider stage
3.
Cryojet controller
located under the mar table
4.
Phi axis
located on the rod to the right of the phi
axis knobs
5.
variable
usually located in the front of 5ID-B
6.
overview
located on the top of the patch panel
7. empty
8. empty
Additionally the output from the ProVideo is also directed to a video card in banana1 (the SGI O2). This allows you to use the program mediarecorder to capture images of whatever is on screen.